What matters
Twitch Emote (28px) dimensions at a glance
The 28×28 emote is the size most viewers actually see in Twitch chat. This is the critical test — if the emote isn't readable here, it doesn't work.
Chat is where emotes live. A beautiful 112px design that becomes an indistinguishable blob at 28px has failed at its primary job.
Fine details, thin lines, and subtle expressions disappear entirely. The emote becomes unrecognizable noise in the chat stream.